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Transplantation of human bone marrow cells into the contused spinal cord |
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We have shown that mesenchymal stem cells derived from bone marrow (bone marrow stromal cells; MSC) result in tissue sparing, provide a permissive environment for axon growth and support recovery of function after transplantation into the injured spinal cord (abstract; Himes et al, in press). |
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MSC in vitro |
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MSC transplanted into a contusion injury fill the injury site, reduce cyst size (see Nissl stain) and support axonal growth (see neurofilament (NF) stain). |
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The proposed mechanism for the axon growth-supporting action of MSC is the creation of a permissive environment via the secretion of growth factors/cytokines. The histogram shows that MSC from 4 tested donors secrete similar factors; however, the amounts (as determined by ELISA) differ among donors. The different growth factor amounts and ratios could potentially effect recovery of function. |
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Rats tranplanted with MSC show faster onset as well as moderately improved recovery of function in the overground locomotion test (BBB) over time compared to control animals. |
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| In this project, we are using multiple translational strategies to test the efficacy of combination treatments and minimally invasive cell-delivery. In addition, we are evaluating if a minimally processed fraction of bone marrow has equally beneficial properties. Outcome measures will include histology to determine effects (neuroprotection, axon re-growth) of the cell transplant on the injury, and behavioral tests to evaluate recovery of motor, sensory and autonomic systems. | ||||
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